Cardiopulmonary Bypass Induces Acute Lung Injury via the High-Mobility Group Box 1/Toll-Like Receptor 4 Pathway
Anti-HMGB1 reduced CPB-induced acute lung injury in rat models. The histological changes of I/R lung injury induced by CPB were evaluated via H&E staining in comparisons of (a) control vs. (b) CPB, (c) anti-HMGB1 vs. (d) anti-HMGB1+CPB, and (e) IgY vs. (f) IgY+CPB. The number of neutrophils, interstitial width, and lung injury score were quantified and are shown in the different groups (g). Compared with the control group, the CPB-only and IgY+CPB groups showed histologic features of lung injury and increased lung injury scores. In the anti-HMGB1+CPB group, however, the lung injury was decreased, and the lung injury scores were lower than those in the CPB-only group. The concentrations of (h) IL-1β and (i) TNF-α were evaluated by ELISAs. The anti-HMGB1 mAb attenuated the CPB-induced increase in IL-1β and TNF-α levels of lung tissues. Data are represented as the , per group. and vs. control; ++ vs. CPB only.