Cardiopulmonary Bypass Induces Acute Lung Injury via the High-Mobility Group Box 1/Toll-Like Receptor 4 Pathway
Knocking down TLR4 could not reduce CPB-induced HMGB1 protein expression in the lung tissues. (a) Western blot results showing TLR4 protein expression in the shNT+CPB, shNT, shTLR4+CPB, shTLR4, CPB, and control groups. (b) The TLR4 protein density relative to that of β-actin. The TLR4 protein density was significantly lower in the shTLR4+CPB group than in the CPB and shNT+CPB groups (a, b). (c) Western blot results showing HMGB1 protein expression in the shNT+CPB, shNT, shTLR4+CPB, shTLR4, CPB, and control groups. (d) The HMGB1 protein density relative to that of β-actin. (e) The concentrations of HMGB1 were evaluated by ELISAs. The reduction of TLR4 by shTLR4 could not reduce the CPB-induced increase in the HMGB1 levels of lung tissues (c–e). Data are represented as the , per group. and vs. control; ++ vs. CPB only.