Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats

Figure 2

The migratory pathway and pattern of NCSCs were observed with Sox10 as a marker. (a) E9, the neural tube has not been completely closed, and the neural crest has not been formed. (b) E9.5, the neural crest began to form, and NCSCs were delaminated. (c) E10, NCSCs migrated symmetrically to both sides along the gap between neural tube and epidermis. (d) E10.5, NCSCs migrated to both sides of salivary glands and chordates. (e) E11, NCSCs migrated to both sides of the dorsal aorta, and some cells entered the bilateral pericardial cavity. (f) E12, NCSCs were transplanted in the pericardial cavity, and some cells began to pass through the original diaphragm and enter the anterior intestine, middle intestine, pancreas, and spleen. (g) E13, NCSCs began to colonize the intestinal wall. (h) E14, ENS is formed. Scale bar, 100 μm.

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