Transneuronal Degeneration in the Visual Pathway of Rats following Acute Retinal Ischemia/Reperfusion

Figure 7

Immunofluorescence images of SYN and PSD-95 expression in the retina after RI/R injury (400x,  μm). I/R-2 h: rats euthanized 2 hours post-RI/R injury; I/R-6 h: rats euthanized 6 hours post-RI/R injury; I/R-12 h: rats euthanized 12 hours post-RI/R injury; I/R-1 d: rats euthanized 1 day post-RI/R injury; I/R-1 d: rats euthanized 3 days post-RI/R injury; I/R-7 d: rats euthanized 7 days post-RI/R injury; I/R-14 d: rats euthanized 14 days post-RI/R injury. SYN: synaptophysin (red); PSD-95: postsynaptic density-95 (green); IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer. The Image J analysis software was used to determine the average gray value of the SYN expression in (A) IPL and (B) OPL, the average gray value of the PSD-95 expression in (C) IPL and (D) OPL. indicates that there were statistical differences when compared with the control groups. indicates that there were significant statistical differences when compared with the control groups.