Transneuronal Degeneration in the Visual Pathway of Rats following Acute Retinal Ischemia/Reperfusion

Figure 8

Western blotting results of SYN and PSD-95 expression in the retina after RI/R injury. (a) 2 h: rats euthanized 2 hours post-RI/R injury; 6 h: rats euthanized 6 hours post-RI/R injury; 12 h: rats euthanized 12 hours post-RI/R injury; 1 d: rats euthanized 1 day post-RI/R injury; 3 d: rats euthanized 3 days post-RI/R injury; 7 d: rats euthanized 7 days. Synaptophysin (SYN), a presynaptic vesicle protein, was significantly increased in the retina at 2 hours to 1 day after RI/R compared to the control group. From 3 days to 14 days, the SYN expression showed a downward trend. (b) PSD-95, a postsynaptic vesicle protein, remains unchanged at 2 hours to 12 hours after RI/R compared to the control group. The PSD-95 expression was significantly reduced in the retina at 1 day to 14 days after RI/R. Use the Image J analysis software to determine the average gray value of the (c) SYN expression and the (d) PSD-95 expression in retina. indicates that there were statistical differences when compared with the control groups. indicates that there were significant statistical differences when compared with the control groups.

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