Cysteine-Rich Intestinal Protein 1 Served as an Epithelial Ovarian Cancer Marker via Promoting Wnt/β-Catenin-Mediated EMT and Tumour Metastasis

Figure 3

Short interfering RNAs (siRNAs) were used to deplete CRIP1 gene expression in vitro. The effect of si-CRIP1 in the OVCAR3 cell line showed no difference in cell proliferation and apoptosis. (a, b) qRT-PCR (a) and western blot (b) were used to detect the gene silencing effect of OVCAR3 cell lines transfected with si-CRIP1 or si-NC. GAPDH and β-actin were used as internal controls. (c) The influence of CRIP1 silencing on cell viability in the OVCAR3 cell line. After transfection with si-CRIP1 or si-NC for 1 day, 2 days, 3 days, and 4 days, cell viability was assessed using CCK8, and OD₄₅₀ was measured. (d, e) The influence of CRIP1 silencing on cell proliferation (d) and apoptosis (e) in the OVCAR3 cell line. After transfection with si-CRIP1 or si-NC, cell proliferation and apoptosis were assessed by the EdU and Annexin V-FITC/PI assays, respectively. Representative graphs are shown on the left. represents the data of three independent experiments. ns: no statistical significance. and .

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