MicroRNA-146-5p Promotes Pulmonary Artery Endothelial Cell Proliferation under Hypoxic Conditions through Regulating USP3

Figure 4

USP3 is a direct binding target gene for miR-146-5p. (a) Schematic representation of miR-146-5p sharing the same complementary seed sequence as the 3-UTR of USP3. (b) Luciferase reporter assay detected that USP3 is the target gene for miR-146-5p. (c) Western blotting was used to detect the protein expression level of USP3 in HPAEC cells after overexpression of miR-146-5p after 48 h of hypoxia. (d) qRT-PCR was used to detect the mRNA expression level of USP3 in HPAEC cells after overexpression of miR-146-5p after 48 h of hypoxia. The data were , and indicates a significant difference compared with the miR-NC group ().

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