Cyclophosphamide Attenuates Fibrosis in Lupus Nephritis by Regulating Mesangial Cell Cycle Progression

Figure 4

(a) Effect of CTX on candidate genes. HMCs were treated with 20 ng/ml PDGF-B for 48 h with or without CTX (40 or 80 mg/l). The expression levels of candidate genes were detected by western blotting and qRT-PCR. Blots were representatives of independent biological triplicates. and . Data are presented as the (). (b) Effect of CTX on cell cycle proteins. CTX (40 and 80 mg/l) upregulated p21 mRNA and protein in HMCs as demonstrated by qRT-PCR and western blotting assays, respectively. The western blots were representatives of independent biological triplicates. and . Data are presented as the ().

(a)

(b)