CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis

Figure 5

LMO4 is a target of miR-637, and circLDLR regulates LMO4 via miR-637 in PTC cells. (a) The predicted binding sites of miR-637 on LMO4 sequences. (b) Luciferase activity detection in TPC-1 and IHH-4 cells cotransfected with the reporter plasmid and the indicated miRNAs using the dual-luciferase reporter assay. (c) qRT-PCR analysis of miR-637 and LMO4 expression in TPC-1 and IHH-4 cells pulled down by Anti-Ago2 or Anti-IgG. (d) Western blot analysis of LMO4 level in TPC-1 and IHH-4 cells transfected with NC, miR-637, circ-NC + miR-637, and circLDLR + miR-637. (e, f) Level detection of LMO4 in 45 PTC tissues and matched normal tissues with qRT-PCR and western blot. (g, h) Correlation analysis between LMO4 and circLDLR or miR-637 in PTC tissues with Pearson correlation analysis. (i) Level detection of LMO4 in PTC cell lines (TPC-1 and IHH-4) and normal Nthy-ori 3-1 cell lines with western blot. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)