Role of Annexin A1 in Squamous Cell Lung Cancer Progression

Figure 2

Correlation between ANXA1 expression and proliferation in SQCLC cell lines. (a) Western blot showing expression of ANXA1 in seven SQCLC cell lines. (b) Expression of ANXA1 in control-siRNA and ANXA1-siRNA treated cell lines. (c) Number of viable cells in ANXA1 compared to control siRNAs -treated cells after 120 hours. (d) Sanger sequencing showing exon seven knockout of ANXA1 gene in the LK2 cell line. (e) Western blot showing expression of ANXA1 protein in wild type and ANXA1 knockout of the LK2 cell line. (f and g) Number of viable cells (48 hours) and cellular proliferation in wild type compared to ANXA1 knockout LK2 cell line, respectively. (h) Overexpression of ANXA1 in the H520 cell line using a mammalian expression vector. (i and k) Number of viable cells (48 hours) and cellular proliferation in empty vector compared to ANXA1 transfected H520 cell lines. All experiments were held with three independent replicates.