Ethacrynic Acid Enhances the Antitumor Effects of Afatinib in EGFR/T790M-Mutated NSCLC by Inhibiting WNT/Beta-Catenin Pathway Activation

Figure 7

The mechanism of EA combined with afatinib had synergistic cytotoxic effects on H1975 cells. (a) The heat map showed gene expression of H1975 from RNA-seq after treatment with afatinib and with or without EA for 24 h. (b) Volcano plot showed the expression of differential genes in afatinib combined EA vs. afatinib alone. (c) Statistics of pathway enrichment on differential expressed genes were analyzed by KEGG (afatinib+EA vs. afatinib). (d) Relative expression of genes in the cancer and cytokine-cytokine receptor interaction pathways. (e) The change of protein expression in H1975 after treatment with afatinib and with or without EA were examined by western blotting.

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