Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro

Figure 2

Effect of ORF7 on the expression of cytokines and chemokines in A549 cells. (a) Relative to control cells, A549-ORF7 cells had increased expression of IL-6, CCL2, and IFN-β (all ), decreased expression of IL-1α, IL-8, and TNF-α (all ), but similar expression of IL-1β and IFN-α (both ). CCL7 and IL-10 were undetectable (qRT-PCR). (b) Activation of innate immunity by SeV/VSV infection increased the expression of CCL2 and decreased the expression of IL-8 in A549-ORF7 cells compared to control cells (both ; qRT-PCR). (c) A549-ORF7 cells had increased levels of CCL2 and decreased levels of IL-8 relative to control cells (both ; ELISA).

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