Validation of a One-Step Reverse Transcription-Droplet Digital PCR (RT-ddPCR) Approach to Detect and Quantify SARS-CoV-2 RNA in Nasopharyngeal Swabs

Table 1

Primers and probes for SARS-CoV-2 detection used in this study.

Target region	Oligonucleotide	Sequence	Reference
E gene	E gene_F	ACAGGTACGTTAATAGTTAATAGCGT	[17]
	E gene_R	ATATTGCAGCAGTACGCACACA
	E gene_P	6-FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ1
RdRP gene	RdRP gene_F	GTGARATGGTCATGTGTGGCGG	[17]
	RdRP gene_R	CARATGTTAAASACACTATTAGCATA
	RdRP gene_P2	6-FAM-CAGGTGGAACCTCATCAGGAGATGC-BHQ1
N gene	N gene_F	CACATTGGCACCCGCAATC	[17]
	N gene_R	GAGGAACGAGAAGAGGCTTG
	N gene_P	6-FAM-ACTTCCTCAAGGAACAACATTGCCA-BHQ1
	N2 gene_F	TTACAAACATTGGCCGCAAA	[18]
	N2 gene_R	GCGCGACATTCCGAAGAA
	N2 gene_P	6-FAM-ACAATTTGCCCCCAGCGCTTCAG-BHQ1
	N3 gene_F	GGGAGCCTTGAATACACCAAAA	[18]
	N3 gene_R	TGTAGCACGATTGCAGCATTG
	N3 gene_P	6-FAM-AYCACATTGGCACCCGCAATCCTG-BHQ1

Y is C/T, R is A/G, and S is C/G.