Mechanism and Role of the Neuropeptide LGI1 Receptor ADAM23 in Regulating Biomarkers of Ferroptosis and Progression of Esophageal Cancer

Figure 4

Overexpression of ADAM23 suppressed tumorigenesis and promoted ferroptosis in esophageal carcinoma cells. (a) Relative expression of ARHGEF26-AS1, miR-372-3p, and ADAM23 in ESCC cells; ; (b) Western blot results showing the expression of the ADAM23, GPX4, SLC7A11, and SLC3A2 proteins in TE-1 cells from the NC, EV, ADAM23-OE, shADMA23-1, and shADAM23-2 groups; ; (c) real-time PCR results showing the mRNA expression levels of ADAM23, GPX4, SLC3A2, and SLC7A11 in TE-1 cells following the overexpression and silencing of ADAM23. ; (d) the effect ADAM23 on cell proliferation, assessed through the CCK-8 assay. ; (e) cell viability and (f) cell death measured in subgroups with different levels of ADAM23 expression ; (g) proliferation and migration of TE-1 cells assessed through the wound healing assay. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)