Mechanism and Role of the Neuropeptide LGI1 Receptor ADAM23 in Regulating Biomarkers of Ferroptosis and Progression of Esophageal Cancer

Figure 6

The ARHGEF26-AS1 lncRNA promoted ferroptosis, inhibited cell growth and upregulated the expression of ADAM23 by sponging miR-372-3p in esophageal carcinoma cells. (a) The binding of miR-372-3p to ARHGEF26-AS1, predicted with RNAhybrid 2.12. (b) Regulation of miR-372-3p by ARHGEF26-AS1, assessed through luciferase reporter assays. vs. miR-NC.; (c) the relative mRNA levels of ADAM23, GPX4, SLC3A2, and SLC7A11 in TE-1 cells cotransfected with the miR-372-3p mimic or shADAM23-1, following the overexpression of ARHGEF26-AS1. Data is presented as the . ; (d) the effect of the miR-372-3p mimic and shADAM23-1 on cell proliferation after the overexpression of ARHGEF26-AS1, assessed through the CCK-8 assay. ; (e, f) the effect of ARHGEF26-AS1 overexpression on (e) cell proliferation and (f) cell death after treatment with either the miR-372-3p mimic or shADAM23-1. ; (g) verification of ARHGEF26-AS1 overexpression through Western blotting following the overexpression of miR-372-3p and the effect of shADAM23-1 on the expression of ADAM23; ; (h) proliferation and migration of TE-1 cells, assessed through the wound healing assay; (i) effect of the ARHGEF26-AS1/miR-372-3p/ADAM23 pathway on ferroptosis. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)