Spike Protein Subunits of SARS-CoV-2 Alter Mitochondrial Metabolism in Human Pulmonary Microvascular Endothelial Cells: Involvement of Factor Xa

Figure 1

(a) Representative Western blot of the factor Xa (FXA) expression in human pulmonary microvascular endothelial cells (HPMEC) incubated in the absence (control) and in the presence of recombinant COVID-19 spike S1 and S2 subunits (10 nmol/L, each subunit; S1+S2 subunits). (b) Representative Western blot of FXA expression in HPMEC under control condition and incubated with LPS (1 μg/mL) with and without recombinant COVID-19 spike S1 and S2 subunits (10 nmol/L, each subunit) (LPS+S1+S2 subunits). Bar graphs show the densitometric analysis of all the Western blots represented as densitometric arbitrary units (AU). Densitometric values are represented as of four experiments. respect to control. ^# respect to LPS.

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