Research Article
[Retracted] Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis
Figure 3
Chlorogenic acid suppressed neuron apoptosis mediated by microglia. The conditioned medium of microglia was harvested and administered to HT-22 cells for 24 hours’ incubation. (a) CCK-8 examined cell proliferation. (b) Western blot confirmed the protein profiles of Bax, C-caspase3, C-caspase8, C-caspase9, and Bcl-2 in cells. (c) Confocal microscopy images displayed the immunofluorescence staining of ROS in HT-22 cells. . (d) The LDH kit was taken to monitor LDH release in cells. Statistics were presented as . . NS , , and (vs. the CMcon group). && and &&& (vs. the CMOGD group).
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