Long Noncoding RNA BCYRN1 Recruits BATF to Promote TM4SF1 Upregulation and Enhance HCC Cell Proliferation and Invasion

Figure 4

BCYRN1 recruits BATF to drive TM4SF1 expression. (a) The impact of BCYRN1 on TM4SF1 promoter activity was examined via the luciferase reporter assay. (b) Interactions between BATF and BCYRN1 were confirmed through a RIP assay, vs. IgG. (c) Putative BATF binding sites within the TM4SF1 promoter. (d) HCCLM3 cells were transfected with BATF expression vectors and truncated TM4SF1 luciferase reporter constructs or (e) mutant TM4SF1 luciferase reporter constructs in a luciferase reporter assay, vs. the OV-NC group. (f) BATF binding to TM4SF1 promoter site 2 was assessed via ChIP assay, vs. IgG. (g) BATF-mediated upregulation of TM4SF1 by BATF following BCYRN1 silencing within HCCLM3 cells. (h) TM4SF1 and BATF levels were assessed by Western blotting, with GAPDH for normalization. Data are from triplicate experiments and were compared via paired -tests when normally distributed.

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