Siglec-15 Silencing Inhibits Cell Proliferation and Promotes Cell Apoptosis by Inhibiting STAT1/STAT3 Signaling in Anaplastic Thyroid Carcinoma

Figure 6

Siglec-15 silencing inhibits the activation of STAT1/STAT3 signaling pathway. Western blot analysis of STAT1 expression, STAT3 expression, cleaved caspase-3 expression, VEGF expression in ARO cells (a), FRO cells (b), and WRO cells (c) in THCA cell treatment with agonistic anti-CD40 mAb or Siglec-15 RNAi. (d) Western blot analysis of STAT1 expression and STAT3 expression in ARO cell transfection with vector-expressing STAT1 or STAT3. Clone formation analysis and flow cytometry analysis of cell proliferation (e) and cell apoptosis (f) in ARO cell transfection with vector-expressing STAT1 and STAT3 and Siglec-15 RNAi. (g) Western blot analysis of cleaved caspase-3 expression and VEGF expression in ARO cell transfection with vector-expressing STAT1 and STAT3 and Siglec-15 RNAi. , ^##, and ^{^^} indicated statistical significance compared with control group, scramble group, or STAT3 group.

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