Research Article
LTB4 Promotes Acute Lung Injury via Upregulating the PLCε-1/TLR4/NF-κB Pathway in One-Lung Ventilation
Figure 5
Inhibition of the PLCε-1/TLR4/NF-KB signaling pathway reduced OLV-induced ALI. (a, b) H&E staining examined the pathology of lung tissues in control rats, OLV rats, PLCε-1 knockdown rats, and okanin-treated rats. (c) The lung W/D ratio of lung tissues in control rats, OLV rats, PLCε-1 knockdown rats, and okanin-treated rats was calculated. (d) The PMN/WBC ratio of BALF in control rats, OLV rats, PLCε-1 knockdown rats, and okanin-treated rats was detected. (e–k) ELISA performed to examine the levels of inflammation mediators (GM-CSF, IL-1β, IL-8, KC, MCP-1, MIP-2, and TNF-α) in control rats, OLV rats, PLCε-1 knockdown rats, and okanin-treated rats. (l–o) Western blot detected the protein levels of Bcl-2, Bax, caspase 3, MLCK, VE-cadherin, occludin, and ZO-1 in control rats, OLV rats, PLCε-1 knockdown rats, and okanin-treated rats. , , and .
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