CDK5 Mediates Proinflammatory Effects of Microglia through Activated DRP1 Phosphorylation in Rat Model of Intracerebral Hemorrhage

Figure 4

Expression of p-DRP1 following ICH is dependent on change of CDK5 kinase activity. Protein expression levels of p-histone H1 (a). Quantification of p-histone H1 after ICH (b). The expression of p-DRP1 (c). Quantification of p-DRP1 after ICH (d). Coimmunoprecipitation between CDK5 and DRP1 in microglia, the upper, IP: DRP1, WB: CDK5, and DRP1; the lower, IP: CDK5, WB: DRP1, and CDK5 (e). The expression level of p-DRP1 following knockdown CDK5 or p35 expression by siRNA (f, g). , vs. sham. ICH: intracerebral hemorrhage; d: day. IP: immunoprecipitation. “Input” means samples before the IP procedure. “IgG” was used to exclude specific binding. “WB” means Western blot.

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