miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis

Figure 1

The effect of TRPV5 on oxalate-induced NRK-52E cells. (a) QRT-PCR was performed to detect the mRNA expression of TRPV5 in NRK-52E cells after TRPV5 overexpression. β-Actin served as the internal control. (b) Western blot was performed to measure the protein level of TRPV5 in NRK-52E cells after TRPV5 overexpression. β-Actin served as the internal control. (c) The expression of TRPV5 in oxalate-induced NRK-52E cells with or without TRPV5 overexpression was detected by qRT-PCR. β-Actin served as the internal control. (d) The protein level of TRPV5 in oxalate-induced NRK-52E cells with or without TRPV5 overexpression was measured by Western blot. β-Actin served as the internal control. (e) MTT assay was conducted to evaluate cell viability. (f) Crystal cell adhesion assay was used to determine and quantify CaOx deposition in specified cells (×200 magnification, scale ). vs. NC; vs. control; and vs. oxalate + NC. TRPV5: transient receptor potential cation channel subfamily V member 5; qRT-PCR: quantitative real-time polymerase chain reaction; MTT: methylthiazolyldiphenyl-tetrazolium bromide; NC: negative control.

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