miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis

Figure 2

The expression of UMOD in oxalate-induced NRK-52E cells and its physiological interaction with TRPV5. (a) QRT-PCR was performed to detect the mRNA expression of UMOD in oxalate-induced NRK-52E cells. β-Actin served as the internal control. (b) Western blot was performed to measure the protein level of UMOD in oxalate-induced NRK-52E cells. β-Actin served as the internal control. (c) CoIP was used to detect the physiological interaction between TRPV5 and UMOD. (d) After UMOD overexpression in NRK-52E cells, qRT-PCR was used to verify the expression of UMOD. β-Actin served as the internal control. (e) The UMOD protein level was verified using Western blot. β-Actin served as the internal control. and vs. control; vs. NC. UMOD: uromodulin; qRT-PCR: quantitative real-time polymerase chain reaction; CoIP: coimmunoprecipitation; NC: negative control.

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