miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis

Figure 4

miR-103a-3p inversely regulated UMOD expression in NRK-52E cells. (a) The TargetScan website was used to predict binding sites between miR-103a-3p and UMOD. (b) Dual-luciferase report assay was carried out to validate that UMOD was targeted by miR-103a-3p. (c) QRT-PCR was performed to detect miR-103a-3p expression following the treatment with the miR-103a-3p inhibitor in NRK-52E cells. U6 was used as the internal control. (d) The effect of the miR-103a-3p inhibitor on UMOD expression in the cells with or without siUMOD was detected by qRT-PCR. β-Actin served as the internal control. (e) The effect of the miR-103a-3p inhibitor on the UMOD protein level in the cells with or without siUMOD was measured by Western blot. β-Actin served as the internal control. vs. MC; vs. IC; and vs. IC + siNC; vs. IC + siUMOD; and vs. I + siNC. UMOD: uromodulin; qRT-PCR: quantitative real-time polymerase chain reaction; NC: negative control; siUMOD: small interfering RNA against UMOD; I: inhibitor; IC: inhibitor control.

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