YTHDF2 Regulates Macrophage Polarization through NF-κB and MAPK Signaling Pathway Inhibition or p53 Degradation

Figure 1

Polarization and YTHDF2 levels in M1/M2 macrophages. (a) LPS+ INF-γ and (b) IL-4 were utilized to treat M0 macrophages for 0, 6, 12, and 24 h, separately. IL-1β, IL-6, IL-10, TNF-α, TGF-β, iNOS, ARG1, and FIZZ levels were analyzed through qRT-PCR, with GAPDH being the endogenous reference. (c, d) IL-6 and TNF-α production in M1 cells, together with IL-10 and TGF-β levels within M2 cells, were detected through ELISA. (e) CD16/32, CD86, CD206, F4/80, and DECTIN-1 expression were determined through FCM in M0/M1/M2 cells. (f) YTHDF2 mRNA level was measured through qRT-PCR following treatment of M0 cells with LPS + INF-γ, with GAPDH being the endogenous reference. (g) YTHDF2 protein expression detected through western blotting after treated M0 cells with LPS+ INF-γ, with β-actin being the endogenous reference. (h) YTHDF2 mRNA expression quantified through qRT-PCR after treated M0 cells with IL-4, with GAPDH being the endogenous reference. (i) YTHDF2 protein expression measured through western blotting after treated M0 cells with IL-4, with β-actin being the endogenous reference. Results are demonstrated by (). ; .

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