YTHDF2 Regulates Macrophage Polarization through NF-κB and MAPK Signaling Pathway Inhibition or p53 Degradation

Figure 2

Role of YTHDF2 silencing in M1/M2 macrophage polarization. (a, b) YTHDF2 silencing efficiency in M0 macrophages was determined through qRT-PCR as well as WB. Mock: transfection reagent-transfected cells; siNC: NC-siRNA-treated cells; () siRNA: YTHDF2 siRNA-treated cells. (c, d) M0 macrophages were subject to transfection using YTHDF2 siRNA (Si) or NC-siRNA (NC), followed by activation using LPS+ INF-γ or IL-4, IL-1β, IL-6, TNF-α, and iNOS levels within M1 cells, together with IL-10, TGF-β, ARG-1, and FIZZ within M2 macrophages were determined through qRT-PCR, with GAPDH being the endogenous reference. (e) IL-6 and TNF-α production in M1 macrophages were determined by ELISA before (NC) or after YTHDF2 knockdown (Si). (f) IL-10 and TGF-β levels within M2 cells were determined by ELISA before (NC) or after YTHDF2 knockdown (Si). (g) CD86 and CD16/32 protein levels within M1 macrophages were detected through FACS before (NC) or after YTHDF2 knockdown (Si). (h) CD206 and DECTIN-1 levels within M2 macrophages were determined through FACS before (NC) or after YTHDF2 knockdown (Si). Data are demonstrated by (). ; .

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