YTHDF2 Regulates Macrophage Polarization through NF-κB and MAPK Signaling Pathway Inhibition or p53 Degradation

Figure 3

The role of p53 in YTHDF2 regulating M1/M2 macrophage polarization. (a, b) p53 mRNA and protein expression were explored through qRT-PCR as well as western blotting within M1 macrophages before and after YTHDF2 knockdown, with GAPDH being the endogenous reference. (c, d) p53 mRNA and protein expression were decided through qRT-PCR as well as western blotting within M2 macrophages before and after YTHDF2 knockdown, with GAPDH being the endogenous reference. (e, f) p53 mRNA and protein expression were detected via qRT-PCR together with WB after stimulation with PFT-α in YTHDF2-silenced M2 macrophages. (g, h) IL-6, IL-10, TNF-α, and ARG1 mRNA levels in YTHDF2-silenced M1/M2 macrophages with or without PFT-α were determined through qRT-PCR, with GAPDH being the endogenous reference. (i, j) IL-6, IL-10, TNF-α, and TGF-β protein levels within YTHDF2-silenced M1/M2 macrophages with or without PFT-α were evaluated through ELISA. Results are revealed by (). ; .

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