YTHDF2 Regulates Macrophage Polarization through NF-<i>κ</i>B and MAPK Signaling Pathway Inhibition or p53 Degradation

<div>Role of YTHDF2 silencing in inflammatory cytokines and p53 mRNA stability in M1/M2 macrophages. M0 macrophages were subject to 24-h transfection using YTHDF2 siRNA (si YTHDF2) or si-NC before M1 (a) or M2 (b) stimulation. Actinomycin D (5 <i>μ</i>g/mL) was then applied for inhibiting mRNA transcription for a 0-, 2-, and 4-h period. mRNA levels of <i>IL-6</i>, <i>IL-10</i>, <i>TNF-α</i>, <i>TGF-β</i>, and <i>P53</i> were determined through qRT-PCR, with <i>GAPDH</i> being the endogenous reference. 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Disease Markers

fig4

Figure 4

Figure 4: YTHDF2 Regulates Macrophage Polarization through NF-<i>κ</i>B and MAPK Signaling Pathway Inhibition or p53 Degradation 

Disease Markers

YTHDF2 Regulates Macrophage Polarization through NF-κB and MAPK Signaling Pathway Inhibition or p53 Degradation

Figure 4