[Retracted] Apelin Alleviates Meniscus Endothelial Cell Apoptosis in Osteoarthritis

Figure 3

DEGs of meniscus endothelial cell subclusters. (a) Left volcano plot shows DEGs of endothelial subcluster EC (HOXA13), and the right volcano plot shows DEGs of subcluster EC (RASGRF2). Data were calculated as OA biopsies against controls. The DEGs with and value <0.05 were considered to be upregulated (red dots), and DEGS with and value <0.05 were considered to be downregulated (blue dots). Genes of interest are indicated in red. (b) qPCR result showing downregulated expression of APLN in meniscus endothelial cells of patients with OA () compared with the controls (). Data represent three similar experiments. . (c) Flow cytometry analysis of surface expression of APLNR on meniscus endothelial cell subclusters. (d) Upper dot plot showing statistical analysis of surface expression of APLNR on meniscus endothelial cell subcluster EC (HOXA13), between controls and patients with OA. The lower dot plot showing statistical analysis of surface expression of APLNR on meniscus endothelial cell subcluster EC (RASGRF2), between the controls () and patients with OA (). Each dot represents a readout. Data represent two similar experiments. . (e) Violin plots showing the expression of intrinsic apoptosis gene (CASP3, BID, and CASP7) and extrinsic apoptosis genes (FAS, TNFRSF1A, and TNFSF10) in endothelial subclusters and in meniscus endothelial cells, between controls and patients with OA.

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