GSK3B Overexpression Alleviates Posttraumatic Osteoarthritis in Mice by Promoting DNMT1-Mediated Hypermethylation of NR4A3 Promoter

Figure 2

GSK3B overexpression alleviates IL-1β-induced degeneration of mouse chondrocytes. IL-1β-induced chondrocytes were transfected with 40 μg/mL pcDNA-GSK3B for 48 h, and then, western blotting was used to detect the expression of GSK3B (a), MMP13, aggrecan, and type II collagen protein (h, i), CCK-8 assay was used to detect cell viability (b), flow cytometry was used to detect apoptosis (c, d), ELISA kits were used to detect the secretion of inflammatory factors TNF-α (e) and IL-6 (f), and RT-qPCR was used to detect the expression of MMP13, aggrecan, and type II collagen mRNA (g). Data is presented as the for six independent experiments and analyzed by Student’s -test or ANOVA test. and .

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