GSK3B Overexpression Alleviates Posttraumatic Osteoarthritis in Mice by Promoting DNMT1-Mediated Hypermethylation of NR4A3 Promoter

Figure 5

NR4A3 interference alleviates IL-1β-induced degeneration of mouse chondrocytes by inhibiting the JAK2/STAT3 pathway. IL-1β-induced chondrocytes were transfected with 200 nM si-NR4A3 or corresponding negative control for 48 h. (a–c, j, k) Western blotting was used to detect the expression of NR4A3, phosphorylated JAK2 (p-JAK2), JAK2, p-STAT3, STAT3, MMP13, aggrecan, and type II collagen proteins. (d) CCK-8 assay was used to detect cell viability. (e, f) Flow cytometry was used to detect apoptosis. (g, h) ELISA kits were used to detect the secretion of inflammatory factors TNF-α and IL-6. (i) RT-qPCR was used to detect the expression of MMP13, aggrecan, and type II collagen mRNA. Data is presented as the for six independent experiments and analyzed by Student’s -test or ANOVA test. and .

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