Ferroptosis-Specific Inhibitor Ferrostatin-1 Relieves H₂O₂-Induced Redox Imbalance in Primary Cardiomyocytes through the Nrf2/ARE Pathway

Figure 2

Ferrostatin-1 inhibited H₂O₂-induced oxidative stress in primary cardiomyocytes. (a) Intracellular ROS levels were detected by flow cytometry. (b) GSH activity was detected by kit. (c) SOD activity was detected by kit. (d) SOD1 and SOD2 protein level was detected by WB. (e) SOD1 mRNA level was detected by qRT-PCR. (f) SOD2 mRNA level was detected by qRT-PCR. (g) The expression of SOD1 was detected by immunofluorescence staining (“” indicates statistical difference from the control group, ; “” indicates statistical difference from the control group, ; “#” indicates statistical difference from the H₂O₂ group, ; “##” indicates statistical difference from the H₂O₂ group, ).

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