Ferroptosis-Specific Inhibitor Ferrostatin-1 Relieves H₂O₂-Induced Redox Imbalance in Primary Cardiomyocytes through the Nrf2/ARE Pathway

Figure 4

Nrf2/ARE pathway was involved in H₂O₂-induced ferroptosis of primary cardiomyocytes. (a) Nrf2, HO-1, and GPX4 protein level was detected by WB. (b) Nrf2 mRNA level was detected by qRT-PCR. (c) HO-1 mRNA level was detected by qRT-PCR. (d) GPX4 mRNA level was detected by qRT-PCR. (e) GPX activity was detected by kit (“” indicates statistical difference from the control group, ; “#” indicates statistical difference from the H₂O₂ group, ; “##” indicates statistical difference from the H₂O₂ group, ).

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