Ferulic Acid Mitigates Growth and Invasion of Esophageal Squamous Cell Carcinoma through Inducing Ferroptotic Cell Death

Figure 3

FA exerts a suppressive effect on migration, invasion, and angiogenesis in ESCC cells. (a–c) The migratory cells were counted when EC-1 and TE-4 cells were administrated with 20 μM, 40 μM, and 60 μM FA for 48 h via Transwell experiment. Scale bar, 100 μm. (d–f) The invasive EC-1 and TE-4 cells with 48 h administration of 20 μM, 40 μM, and 60 μM FA were counted through Transwell experiment. Scale bar, 100 μm. (g, h) VEGFA mRNA level was measured in EC-1 and TE-4 cells that were administrated with 20 μM, 40 μM, and 60 μM FA for 48 h utilizing quantitative RT-PCR analysis. (i, j) PDGFB mRNA level was tested in EC-1 and TE-4 cells with 48 h administration of 20 μM, 40 μM, and 60 μM FA through adopting quantitative RT-PCR analysis. was computed through one-way ANOVA test. Significance level was denoted as , , , and .

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