Ferulic Acid Mitigates Growth and Invasion of Esophageal Squamous Cell Carcinoma through Inducing Ferroptotic Cell Death

Figure 6

DFO impairs the stimulative effect of FA on lipid peroxidation and ROS generation in ESCC cells. (a, b) MDA content was measured in EC-1 and TE-4 cells with 48 h administration of 40 μM FA and 50 μM DFO by MDA content kit. (c, d) SOD activity was tested in EC-1 and TE-4 cells that were administrated with 40 μM FA and 50 μM DFO for 48 h through adopting SOD activity kit. (e, f) GSH content was assayed in EC-1 and TE-4 cells under exposure to 40 μM FA and 50 μM DFO for 48 h. (g–i) Intracellular ROS accumulation was assessed in EC-1 and TE-4 cells with 48 h administration of 40 μM FA and 50 μM DFO via DCFH-DA probe. Scale bar, 20 μm. was computed through one-way ANOVA test. Significance level was denoted as , , and .

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