Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an In Vitro Model of Actinic Dermatitis by Ultraviolet

Figure 5

Knockdown of SPAG5 ameliorated UV irradiation-mediated dysfunction of keratinocytes. (a) The si-NC and si-SPAG5 were transfected into HaCaT cells and the expression of SPAG5 was evaluated by RT-PCR 24 hours later. We then exposed the transfected HaCaT cells to UV irradiation and treated them with 5 μM curcumin for 24 hours. (b) UV-irradiated HaCaT cell proliferation was assayed with CCK-8. (c)–(f) The levels of SOD, GSH-PX, MDA, and ROS in UV-irradiated HaCaT cells were tested using an oxidative stress assay kit and cytofluorimetry. (g) Expression of inflammatory factors (IL-1β, IL-6, IL-18, and TNFα) in UV-irradiated HaCaT cells was checked by RT-PCR. (h) Expression of apoptosis-related proteins (Bax, Bcl-xL, Caspase3, Caspase8, Caspase9) in UV-irradiated HaCaT cells was detected by WB. (i) WB was implemented to testify the profiles of oxidative stress-related proteins (Keap1, Nrf2, HO-1, COX2, and iNOS) in UV-irradiated HaCaT cells. (j) The expression of inflammatory response proteins (NF-κB, MMP1, and MMP9) in UV-irradiated HaCaT cells was evaluated by WB. (k) The SPAG5/FOXM1 pathway expression in UV-irradiated HaCaT cells was testified by WB. . (vs. si-NC or UV group), , ; ^# (vs. si-SPAG5+UV group), ^##, ^###.

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