Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an In Vitro Model of Actinic Dermatitis by Ultraviolet

Figure 6

Knockdown of FOXM1 alleviated UV irradiation-mediated dysfunction of keratinocytes. (a) si-NC and si-FOXM1 were transfected into HaCaT cells and transfection efficiency was measured by RT-PCR after 24 hours. The transfected HaCaT cells were processed by UV irradiation and then treated with curcumin (5 μM) for 24 hours. (b) CCK-8 was employed to examine the proliferation of UV-irradiated HaCaT cells. (c)–(e) The contents of SOD, GSH-PX, and MDA in UV-irradiated HaCaT cells were evaluated using the Oxidative Stress Assay Kit. (f) Cytofluorimetry was applied to assay the level of ROS in UV-irradiated HaCaT cells. (g) The expression of inflammatory cytokines in UV-irradiated HaCaT cells was monitored by RT-PCR. (h) WB was adopted to verify the expression of apoptosis-related proteins in UV-irradiated HaCaT cells. (i) WB gauged the expression of oxidative stress-related proteins in UV-irradiated HaCaT cells. (j) The profiles of inflammatory response proteins in UV-irradiated HaCaT cells were compared by WB. (k) WB assessed the SPAG5/FOXM1 pathway expression in UV-irradiated HaCaT cells. . (vs. si-NC or UV group), , ; ^# (vs. si-FOXM1+UV group), ^##, ^###.

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