circ_000166/miR-296 Aggravates the Process of Diabetic Renal Fibrosis by Regulating the SGLT2 Signaling Pathway in Renal Tubular Epithelial Cells

Figure 5

SGLT2 as a target gene regulated by miR-296. (a, b) We used qRT-PCR to detect the expression of SGLT2 in the kidney tissue and the cells of each group. High-glucose group (high-glucose medium containing D-glucose: 25 mmol/L). (c, d QRT-PCR was used to detect the effect of the miR-296 mimic and miR-296 inhibitor on the expression of SGLT2. (e, f) QRT-PCR and western blotting were used to detect the effect of inhibiting circ_000166 on the expression of SGLT2. The data are expressed as . , , and .

(a)

(b)

(c)

(d)

(e)

(f)