Knockdown of ILK Alleviates High Glucose-Induced Damage of H9C2 Cells through TLR4/MyD88/NF-κB Pathway

Figure 1

High glucose damages H9C2 cells. (a) The cell activity of H9C2 was determined by MTT assay. (b–e) The levels of SOD, CAT, GSH-Px, and MDA in the supernatant were determined by the kits. (f–h) The levels of MPO, IL-1β, and IL-8 in the supernatant were determined by the ELISA. (i) Tunel staining was performed to examine the apoptosis level of H9C2. (j) mRNA expression results of ILK were determined by real-time PCR. (“” indicates statistical difference from the control group .).

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