Knockdown of ILK Alleviates High Glucose-Induced Damage of H9C2 Cells through TLR4/MyD88/NF-κB Pathway

Figure 3

Knocking down ILK can improve H9C2 inflammatory response induced by high glucose. (a) Immunofluorescence was performed to examine the expression of IL-α. (b) Protein expression results of IL-1β were determined by Western blotting. β-Actin was used as an internal control. (c–e) The levels of MPO, IL-1β, and IL-8 in the supernatant were determined by the ELISA. (f–h) mRNA expression results of MPO, IL-1β, and IL-8 were determined by real-time PCR. (“” indicates statistical difference from the control group , and “#” indicates statistical difference from the HG group .).

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