Prognostic Biomarkers Identification in Esophageal Cancer Based on WGCNA and Single-Cell Analysis

Figure 9

Subpopulation identification and pseudotime trajectory analysis in macrophages. (a) tSNE plot of macrophage cluster. (b) Cell annotation map with marker genes. (c) The differentiation of macrophages in tumor tissue and adjacent tissue. (d) Diagram of pseudotime trajectory differentiation stages of macrophages. (e) Pseudotime trajectory analysis differentiation plot based on cell types. (f) Status of macrophages differentiation by pseudotime trajectory analysis. (g) Pseudotime trajectory differentiation map of macrophages in tumor tissues and adjacent tissue. (h) The heat map of the gene expression during macrophage differentiation. (i) The expression of hub genes during the differentiation of Macrophages_M1_like and Macrophages_M2_like subsets. (j) Macrophages are divided into positive group and negative group according to whether FOS gene is expressed. (k) GO function enrichment results of FOS⁺ Macrophages cells upregulated genes.

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