Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Figure 4

Immunofluorescence was used to detect the impact of local injection of DEX around the sciatic nerve on SNPH, KIF5B, Drp-1, and OPA1 expressions. (a) Immunofluorescence localization showed that local injection of DEX around the sciatic nerve significantly increased the number of SNPH and (g) OPA1 while lowering the number of (c) KIF5B- and (e) Drp-1-positive cells in the distal nerve after CCI. Photographs were taken at ×100 magnification. The scale presents a length of 50 μm. The bar graph represents the immunohistochemical scores of various groups of (b) SNPH, (d) KIF5B, (f) Drp-1, and (h) OPA1. Data are () and denote three independent experiments. versus negative control group; ^### versus CCI group; ^$$ and ^$$$ CCI+DEX versus CCI+DEX+CC group.

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