Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Figure 5

The impact of local injection of DEX around the sciatic nerve on the expressions of SNPH, KIF5B, Drp-1, and OPA1 was identified by the western blot analysis. (a) Western blot showed that the local injection of DEX around the sciatic nerve significantly enhanced the expression of SNPH and OPA1 and lowered the expression of KIF5B and Drp-1 in the distal nerve after CCI. Densitometric quantification of (b) SNPH/GAPDH, (c) KIF5B/GAPDH, (d) Drp-1/GAPDH, and (e) OPA1/GAPDH. Data are () and represent three independent experiments. versus negative control group; ^# and ^### versus CCI group; ^$ and ^$$ CCI+DEX versus CCI+DEX+CC group.

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