Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Figure 6

Effects of DEX on neuron-specific marker protein c-Fos and inflammatory mediators in the DHSC. (a) Immunofluorescence localization showed that local injection of DEX around the sciatic nerve significantly decreased the number of c-Fos-positive cells in the DHSC after CCI injuries. Photographs were taken at ×100 magnification. The scale shows a length of 50 μm. (b) Bar graph represents immunohistochemical scores of various groups. (c) Western blot was used to detect the expression of c-Fos in the DHSC. (d) Densitometric quantification of c-Fos/GAPDH. Levels of (e) TNF-α and (f) IL-6 in the DHSC. Data are () and represent three independent experiments. and versus negative control group; ^## and ^### versus CCI group; ^$ and ^$$ CCI+DEX versus CCI+DEX+CC group.

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