Research Article

[Retracted] Influenza A Virus Production in Mouse Lung Is Inhibited by Inhalation of Aerosol Polyethylenimine/Short Hairpin RNA Plasmid Complexes

Figure 1

Recombinant expression plasmid construction. (a) pLKD-CMV-G&PR-U6-shRNA was digested with AgeI and EcoRI to obtain a 8.2 kb vector fragment and the 312 bp ccdB gene. Lane 1, undigested vector; lane 2, vector digested with AgeI and EcoRI; lane 3, DNA ladder marker: 10, 8, 6, 5, 4, 3.5, 3, 2.5, 2, 1.5, and 1 kb and 75, 500, and 250 bp. (b) Transformants were identified by colony PCR. Lane 1, negative control (water); lane 2, negative control (mock plasmid; 586 bp); lane 3, DL2000 DNA marker; lanes 4–11, positive clones (332 bp).