Effects of Escin on Oxidative Stress and Apoptosis of H9c2 Cells Induced by H₂O₂

Figure 4

Escin inhibited inflammation of H9c2 cells. (a) The expression of IL-1β and TNF-α in the H₂O₂ treatment group increased significantly but decreased in the H₂O₂+escin group. (b) Statistical results of expression of TNF-α ( vs. control; ^# vs. H₂O₂, ). (c) Statistical results of expression of IL-1β ( vs. control; ^# vs. H₂O₂, ). (d) TNF-α mRNA expression was similar to the results of Western blot ( vs. control; ^# vs. H₂O₂, ). (e) IL-1β mRNA expression was also similar to the results of Western blot ( vs. control; ^# vs. H₂O₂, ). (f) Immunofluorescence showed that escin significantly reduced H₂O₂-mediated elevation of IL-1β (magnification: ×400).

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