circIFT80 Functions as a ceRNA for miR-142, miR-568, and miR-634 and Promotes the Progression of Colorectal Cancer by Targeting β-Catenin

Figure 6

circIFT80 acted as a molecular sponge for miR-142, miR-568, and miR-634 in CRC cells. (a) circIFT80 containing wild-type (WT) or mutant (MUT) binding sites along with the sequence complementarity between miR-142, miR-634, and miR-568. (b) Relative luciferase activity was detected in 293 T cells after cotransfection with the WT or MUT circIFT80 reporter plasmids in NC or miR-142, miR-634, and miR-568 mimic. (c) circIFT80 expression levels were analyzed by qPCR after RNA pull-down assays using biotin-labeled miR-142, miR-568, and miR-634 probes and a control probe. (d) In situ hybridization assay of the colocation of circIFT80 and miR-142, miR-568, and miR-634. (e) The expression level of miR-142, miR-568, and miR-634 between CRC cells (HT-29 and SW480) and normal human colonic epithelial cells (HcoEpic) was analyzed by qPCR assay. (f) The expression level of miR-142, miR-568, and miR-634 between CRC tissues and tumor adjacent normal tissues was detected by qPCR assay. (g) The cell viability of HT-29 cells following different transfections, as evaluated by CCK-8 assays. U6 was used as an internal control. .

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