circIFT80 Functions as a ceRNA for miR-142, miR-568, and miR-634 and Promotes the Progression of Colorectal Cancer by Targeting β-Catenin

Figure 7

β-Catenin was a direct target of miR-142, miR-568, and miR-634. (a) The 3UTR regions of CTNNB1 (β-catenin mRNA) containing wild-type (WT) or mutant (MUT) binding sites along with the sequence complementarity between miR-142, miR-634, and miR-568. (b) Relative luciferase activity was detected in 293 T cells after cotransfection with WT or MUT CTNNB1 reporter plasmids in NC or miR-142, miR-634, and miR-568 mimic. (c) CTNNB1 expression levels were analyzed by qPCR after RNA pull-down assays with biotin-labeled miR-142, miR-568, and miR-634 probes and a control probe. (d) The mRNA relative expression level of β-catenin in CRC and adjacent normal tissues in GEPIA downloaded from the TCGA. (e) β-Catenin expression in six pairs of CRC tissues and corresponding tumor adjacent normal tissues as detected by IHC assays. Quantification analysis is shown in the right panel. (f, g) The protein levels of β-catenin and c-myc were analyzed by western blotting in HT-29 cells following different transfections. The scale bar represents 100 μm. .

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