[Retracted] α-Cyperone Protects Cardiomyocytes against Oxygen-Glucose Deprivation-Induced Inflammation and Oxidative Stress by Akt/FOXO3a/NF-κB Pathway

Figure 6

NF-κB inhibition counteracted the protective effect of α-cyperone on OGD-mediated myocardial injury. After treatment of OGD-induced cardiomyocytes with α-cyperone (60 μM) for 24 hours, the NF-κB inhibitor Bay 11-7082 was added. (a) Cardiomyocyte viability was assayed with CCK-8. (b) Cardiomyocyte apoptosis was examined by flow cytometry. (c) Expression of apoptosis-associated proteins was gauged by WB. (d) qRT-PCR assessed the relative expression of inflammatory factors. (e) Expression of inflammatory response proteins was monitored by WB. (f) MDA levels and SOD activity were checked by the colorimetric assay. (g) ROS production was gauged using a ROS assay kit. All experiments were repeated three times and data are expressed as ; (vs. OGD), , and .

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