Circulating Exosomal miR-144-3p from Crohn’s Disease Patients Inhibits Human Umbilical Vein Endothelial Cell Function by Targeting FN1

Figure 5

miR-144-3p affects HUVEC viability, migration, and angiogenesis. CY3 fluorescently labeled miR-144-3p-transfected HUVECs and the relative expression of miR-144-3p in miR-144-3p mimic-transfected cells and mimic NC-transfected cells (a). The viability of different transfected cells was measured by CCK-8 assays (b). Wound healing analyses of HUVECs after different transfections and representative images of the extent of cell migration into the wounded area are shown (c). The indicated cells travelled through the membrane during the Transwell invasion assay. Representative images of the Transwell assay results (d). The angiogenesis of the treated cells was evaluated by the tube formation assay (e); , , and .

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